@article{Ruizendaal2017-lk,

title = {Presence of quintuple dhfr N51, C59, S108 - dhps A437, K540  mutations in Plasmodium falciparum isolates from pregnant women  and the general population in Nanoro, Burkina Faso},

author = {Esm\'{e}e Ruizendaal and Marc C Tahita and Maminata Traor\'{e}-Coulibaly and Halidou Tinto and Henk D F H Schallig and Petra F Mens},

doi = {10.1016/j.molbiopara.2017.08.003},

year  = {2017},

date = {2017-10-01},

urldate = {2017-10-01},

journal = {Mol. Biochem. Parasitol.},

volume = {217},

pages = {13--15},

abstract = {Sulphadoxine-pyrimethamine (SP) is only used for intermittent 

 preventive treatment during pregnancy (IPTp) in most Sub-Saharan 

 African countries. However, there are concerns about the efficacy 

 of IPTp-SP because of increasing resistance. Combinations of 

 point mutations in the dhps and dhfr genes of Plasmodium 

 falciparum are associated with SP resistance, in particular the 

 quintuple dhfr (N51, C59, S108) - dhps (codons A437, K540) 

 mutant. In Nanoro, Burkina Faso, filter paper samples from 

 pregnant women at first antenatal care visit and at delivery plus 

 samples from the general population (GP) were studied for dhfr 

 and dhps mutations by sequencing. Quintuple mutants were present 

 in 2 delivery and 4 GP samples. This is the first time the 

 quintuple mutation is found in Burkina Faso and although the 

 prevalence is still very low, emergence of the quintuple mutation 

 could highly diminish the efficacy of IPTp-SP. Close surveillance 

 of SP resistance mutations is therefore warranted.},

keywords = {Intermittent preventive treatment; Plasmodium falciparum;   Pregnancy; Resistance; Sulphadoxine-pyrimethamine},

pubstate = {published},

tppubtype = {article}

}