 | Esmée Ruizendaal, Marc C Tahita, Ronald B Geskus, Inge Versteeg, Susana Scott, Umberto d’Alessandro, Palpouguini Lompo, Karim Derra, Maminata Traore-Coulibaly, Menno D Jong, Henk D F H Schallig, Halidou Tinto, Petra F Mens Increase in the prevalence of mutations associated with sulfadoxine-pyrimethamine resistance in Plasmodium falciparum isolates collected from early to late pregnancy in Nanoro, Burkina Faso (Journal Article) In: Malar. J., vol. 16, no. 1, pp. 179, 2017, ISSN: 1475-2875. @article{Ruizendaal2017-zh,
title = {Increase in the prevalence of mutations associated with sulfadoxine-pyrimethamine resistance in Plasmodium falciparum isolates collected from early to late pregnancy in Nanoro, Burkina Faso},
author = {Esm\'{e}e Ruizendaal and Marc C Tahita and Ronald B Geskus and Inge Versteeg and Susana Scott and Umberto d'Alessandro and Palpouguini Lompo and Karim Derra and Maminata Traore-Coulibaly and Menno D Jong and Henk D F H Schallig and Halidou Tinto and Petra F Mens},
doi = {10.1186/s12936-017-1831-y},
issn = {1475-2875},
year = {2017},
date = {2017-04-01},
urldate = {2017-04-01},
journal = {Malar. J.},
volume = {16},
number = {1},
pages = {179},
abstract = {BACKGROUND: Pregnant women are a high-risk group for Plasmodium
falciparum infections, which may result in maternal anaemia and
low birth weight newborns, among other adverse birth outcomes.
Intermittent preventive treatment with sulfadoxine-pyrimethamine
during pregnancy (IPTp-SP) is widely implemented to prevent these
negative effects of malaria. However, resistance against SP by P.
falciparum may decrease efficacy of IPTp-SP. Combinations of
point mutations in the dhps (codons A437, K540) and dhfr genes
(codons N51, C59, S108) of P. falciparum are associated with SP
resistance. In this study the prevalence of SP resistance
mutations was determined among P. falciparum found in pregnant
women and the general population (GP) from Nanoro, Burkina Faso
and the association of IPTp-SP dosing and other variables with
mutations was studied. METHODS: Blood spots on filter papers were
collected from pregnant women at their first antenatal care visit
(ANC booking) and at delivery, from an ongoing trial and from the
GP in a cross-sectional survey. The dhps and dhfr genes were
amplified by nested PCR and products were sequenced to identify mutations conferring resistance (ANC bookin},
keywords = {Mutations; Plasmodium falciparum; Pregnancy; Resistance; Sulfadoxine-pyrimethamine},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: Pregnant women are a high-risk group for Plasmodium
falciparum infections, which may result in maternal anaemia and
low birth weight newborns, among other adverse birth outcomes.
Intermittent preventive treatment with sulfadoxine-pyrimethamine
during pregnancy (IPTp-SP) is widely implemented to prevent these
negative effects of malaria. However, resistance against SP by P.
falciparum may decrease efficacy of IPTp-SP. Combinations of
point mutations in the dhps (codons A437, K540) and dhfr genes
(codons N51, C59, S108) of P. falciparum are associated with SP
resistance. In this study the prevalence of SP resistance
mutations was determined among P. falciparum found in pregnant
women and the general population (GP) from Nanoro, Burkina Faso
and the association of IPTp-SP dosing and other variables with
mutations was studied. METHODS: Blood spots on filter papers were
collected from pregnant women at their first antenatal care visit
(ANC booking) and at delivery, from an ongoing trial and from the
GP in a cross-sectional survey. The dhps and dhfr genes were
amplified by nested PCR and products were sequenced to identify mutations conferring resistance (ANC bookin |
