| Paul Sondo, Biebo Bihoun, Marc Christian Tahita, Karim Derra, Toussaint Rouamba, Seydou Nakanabo Diallo, Adama Kazienga, Hamidou Ilboudo, Innocent Valea, Zekiba Tarnagda, Hermann Sorgho, Thierry Lef`evre, Halidou Tinto Plasmodium falciparum gametocyte carriage in symptomatic patients shows significant association with genetically diverse infections, anaemia, and asexual stage density (Journal Article) In: Malar. J., vol. 20, no. 1, pp. 31, 2021, ISSN: 1475-2875, (PMID: 33413393
PMCID: PMC7791700). @article{Sondo2021-at,
title = {Plasmodium falciparum gametocyte carriage in symptomatic patients shows significant association with genetically diverse infections, anaemia, and asexual stage density},
author = {Paul Sondo and Biebo Bihoun and Marc Christian Tahita and Karim Derra and Toussaint Rouamba and Seydou Nakanabo Diallo and Adama Kazienga and Hamidou Ilboudo and Innocent Valea and Zekiba Tarnagda and Hermann Sorgho and Thierry Lef`evre and Halidou Tinto},
doi = {10.1186/s12936-020-03559-0},
issn = {1475-2875},
year = {2021},
date = {2021-01-07},
urldate = {2021-01-01},
journal = {Malar. J.},
volume = {20},
number = {1},
pages = {31},
publisher = {Springer Science and Business Media LLC},
abstract = {BACKGROUND: Multi-genotype malaria infections are frequent in
endemic area, and people commonly harbour several genetically
distinct Plasmodium falciparum variants. The influence of
genetic multiplicity and whether some specific genetic variants
are more or less likely to invest into gametocyte production is
not clearly understood. This study explored host and
parasite-related risk factors for gametocyte carriage, and the
extent to which some specific P. falciparum genetic variants are
associated with gametocyte carriage. METHODS: Gametocytes and
asexual forms were detected by light microscopy on thick smears
collected between 2010 and 2012 in Nanoro, Burkina Faso.
Merozoite surface protein 1 and 2 were genotyped by nested PCR
on clinical samples. Associations between gametocyte carriage
and factors, including multiplicity of infection, parasite
density, patient age, gender, haemoglobin (Hb) level, and body
temperature were assessed. The relationship between the presence
of a particular msp1 and msp2 genetic variants and gametocyte
carriage was also explored. RESULTS: Of the 724 samples positive
to P. falciparum and successfully genotyped, gametocytes were
found in 48 samples (6.63%). There was no effect of patient
gender, age and body temperature on gametocyte carriage.
However, the probability of gametocyte carriage significantly
increased with increasing values of multiplicity of infection
(MOI). Furthermore, there was a negative association between
parasite density and gametocyte carriage. MOI decreased with
parasite density in gametocyte-negative patients, but increased
in gametocyte carriers. The probability of gametocyte carriage
decreased with Hb level. Finally, the genetic composition of the
infection influenced gametocyte carriage. In particular, the
presence of RO33 increased the odds of developing gametocytes by
2 while the other allelic families K1, MAD20, FC27, and 3D7 had
no significant impact on the occurrence of gametocytes in
infected patients. CONCLUSION: This study provides insight into
potential factors influencing gametocyte production in
symptomatic patients. The findings contribute to enhance
understanding of risk factors associated with gametocyte
carriage in humans. Trial registration NCT01232530.},
note = {PMID: 33413393
PMCID: PMC7791700},
keywords = {Anemia/epidemiology/parasitology, Burkina Faso/epidemiology, Falciparum/epidemiology/parasitology, Gametocyte, Humans, Malaria, msp1, msp2, Multiplicity of infection, Plasmodium falciparum, Plasmodium falciparum/physiology},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: Multi-genotype malaria infections are frequent in
endemic area, and people commonly harbour several genetically
distinct Plasmodium falciparum variants. The influence of
genetic multiplicity and whether some specific genetic variants
are more or less likely to invest into gametocyte production is
not clearly understood. This study explored host and
parasite-related risk factors for gametocyte carriage, and the
extent to which some specific P. falciparum genetic variants are
associated with gametocyte carriage. METHODS: Gametocytes and
asexual forms were detected by light microscopy on thick smears
collected between 2010 and 2012 in Nanoro, Burkina Faso.
Merozoite surface protein 1 and 2 were genotyped by nested PCR
on clinical samples. Associations between gametocyte carriage
and factors, including multiplicity of infection, parasite
density, patient age, gender, haemoglobin (Hb) level, and body
temperature were assessed. The relationship between the presence
of a particular msp1 and msp2 genetic variants and gametocyte
carriage was also explored. RESULTS: Of the 724 samples positive
to P. falciparum and successfully genotyped, gametocytes were
found in 48 samples (6.63%). There was no effect of patient
gender, age and body temperature on gametocyte carriage.
However, the probability of gametocyte carriage significantly
increased with increasing values of multiplicity of infection
(MOI). Furthermore, there was a negative association between
parasite density and gametocyte carriage. MOI decreased with
parasite density in gametocyte-negative patients, but increased
in gametocyte carriers. The probability of gametocyte carriage
decreased with Hb level. Finally, the genetic composition of the
infection influenced gametocyte carriage. In particular, the
presence of RO33 increased the odds of developing gametocytes by
2 while the other allelic families K1, MAD20, FC27, and 3D7 had
no significant impact on the occurrence of gametocytes in
infected patients. CONCLUSION: This study provides insight into
potential factors influencing gametocyte production in
symptomatic patients. The findings contribute to enhance
understanding of risk factors associated with gametocyte
carriage in humans. Trial registration NCT01232530. |