Paul Sondo, Biebo Bihoun, Bérenger Kabore, Marc Christian Tahita, Karim Derra, Toussaint Rouamba, Seydou Nakanabo Diallo, Adama Kazienga, Hamidou Ilboudo, Innocent Valea, Zekiba Tarnagda, Hermann Sorgho, Thierry Lefevre, Halidou Tinto
In: Pan Afr. Med. J., vol. 39, pp. 118, 2021, ISSN: 1937-8688, (Copyright: Paul Sondo et al. PMID: 34512854 PMCID: PMC8396377).
(Tags: Antimalarials/pharmacology, Burkina Faso, Drug Resistance, Falciparum/drug therapy/parasitology, GeneticRestriction Fragment Length, Genotype, Humans, Malaria, Membrane Transport Proteins/genetics, msp1, msp2, Multidrug Resistance-Associated Proteins/genetics, Mutation, Pfcrt, Pfmdr1, Plasmodium falciparum, Plasmodium falciparum/drug effects/genetics/isolation & purification, Polymerase Chain Reaction, Polymorphism, Protozoan Proteins/genetics)| | | |
Introduction: from a genetic point of view P. falciparumis
extremely polymorphic. There is a variety of parasite strains
infesting individuals living in malaria endemic areas. The
purpose of this study is to investigate the relationship between
polymorphisms in Plasmodium falciparum parasites and Pfcrt and
Pfmdr1 gene mutations in Nanoro area, Burkina Faso. Methods:
blood samples from plasmodium carriers residing in the Nanoro
Health District were genotyped using nested PCR. Parasite gene
mutations associated with resistance to antimalarial drugs were
detected by PCR-RFLP. Results: samples of 672 patients were
successfully genotyped. No msp1and msp2allelic families
exhibited an increase in developing mutations in resistance
genes. However, mutant strains of these genes were present at
greater levels in monoclonal infections than in multi-clonal
infections. Conclusion: this study provides an overview of the
relationship between polymorphisms in Plasmodium falciparum
parasites and mutations in resistance genes. These data will
undoubtedly contribute to improving knowledge of the parasite´s
biology and its mechanisms of resistance to antimalarial drugs.
Paul Sondo, Biebo Bihoun, Marc Christian Tahita, Karim Derra, Toussaint Rouamba, Seydou Nakanabo Diallo, Adama Kazienga, Hamidou Ilboudo, Innocent Valea, Zekiba Tarnagda, Hermann Sorgho, Thierry Lef`evre, Halidou Tinto
In: Malar. J., vol. 20, no. 1, pp. 31, 2021, ISSN: 1475-2875, (PMID: 33413393 PMCID: PMC7791700).
(Tags: Anemia/epidemiology/parasitology, Burkina Faso/epidemiology, Falciparum/epidemiology/parasitology, Gametocyte, Humans, Malaria, msp1, msp2, Multiplicity of infection, Plasmodium falciparum, Plasmodium falciparum/physiology)| | | |
BACKGROUND: Multi-genotype malaria infections are frequent in
endemic area, and people commonly harbour several genetically
distinct Plasmodium falciparum variants. The influence of
genetic multiplicity and whether some specific genetic variants
are more or less likely to invest into gametocyte production is
not clearly understood. This study explored host and
parasite-related risk factors for gametocyte carriage, and the
extent to which some specific P. falciparum genetic variants are
associated with gametocyte carriage. METHODS: Gametocytes and
asexual forms were detected by light microscopy on thick smears
collected between 2010 and 2012 in Nanoro, Burkina Faso.
Merozoite surface protein 1 and 2 were genotyped by nested PCR
on clinical samples. Associations between gametocyte carriage
and factors, including multiplicity of infection, parasite
density, patient age, gender, haemoglobin (Hb) level, and body
temperature were assessed. The relationship between the presence
of a particular msp1 and msp2 genetic variants and gametocyte
carriage was also explored. RESULTS: Of the 724 samples positive
to P. falciparum and successfully genotyped, gametocytes were
found in 48 samples (6.63%). There was no effect of patient
gender, age and body temperature on gametocyte carriage.
However, the probability of gametocyte carriage significantly
increased with increasing values of multiplicity of infection
(MOI). Furthermore, there was a negative association between
parasite density and gametocyte carriage. MOI decreased with
parasite density in gametocyte-negative patients, but increased
in gametocyte carriers. The probability of gametocyte carriage
decreased with Hb level. Finally, the genetic composition of the
infection influenced gametocyte carriage. In particular, the
presence of RO33 increased the odds of developing gametocytes by
2 while the other allelic families K1, MAD20, FC27, and 3D7 had
no significant impact on the occurrence of gametocytes in
infected patients. CONCLUSION: This study provides insight into
potential factors influencing gametocyte production in
symptomatic patients. The findings contribute to enhance
understanding of risk factors associated with gametocyte
carriage in humans. Trial registration NCT01232530.
Paul Sondo, Karim Derra, Toussaint Rouamba, Seydou Nakanabo Diallo, Paul Taconet, Adama Kazienga, Hamidou Ilboudo, Marc Christian Tahita, Innocent Valéa, Hermann Sorgho, Thierry Lefèvre, Halidou Tinto
Determinants of Plasmodium falciparum multiplicity of infection and genetic diversity in Burkina Faso. (Journal Article)
In: Parasites & vectors, vol. 13, iss. 1, pp. 427, 2020.
(Tags: Age Factors, Antigens, Burkina Faso/epidemiology, Falciparum/epidemiology/parasitology, Genetic Variation, Genotype, Humans, Incidence, Malaria, Merozoite Surface Protein 1/genetics, msp1, msp2, Multiplicity of infection, Parasite Load, Plasmodium falciparum, Plasmodium falciparum/*genetics, Protozoan Proteins/genetics, Protozoan/genetics, Seasons)| |
BACKGROUND: Investigating malaria transmission dynamics is essential to inform policy decision making. Whether multiplicity of infection (MOI) dynamic from individual infections could be a reliable malaria metric in high transmission settings with marked variation in seasons of malaria transmission has been poorly assessed. This study aimed at investigating factors driving Plasmodium falciparum MOI and genetic diversity in a hyperendemic area of Burkina Faso. METHODS: Blood samples collected from a pharmacovigilance trial were used for polymerase chain reaction genotyping of the merozoite surface proteins 1 and 2. MOI was defined as the number of distinct parasite genotypes co-existing within a particular infection. Monthly rainfall data were obtained from satellite data of the Global Precipitation Measurement Database while monthly malaria incidence aggregated data were extracted from District Health Information Software 2 medical data of the Center-West health regional direction. RESULTS: In the study area, infected people harboured an average of 2.732 (± 0.056) different parasite genotypes. A significant correlation between the monthly MOI and the monthly malaria incidence was observed, suggesting that MOI could be a good predictor of transmission intensity. A strong effect of season on MOI was observed, with infected patients harbouring higher number of parasite genotypes during the rainy season as compared to the dry season. There was a negative relationship between MOI and host age. In addition, MOI decreased with increasing parasite densities, suggesting that there was a within-host competition among co-infecting genetically distinct P. falciparum variants. Each allelic family of the msp1 and msp2 genes was present all year round with no significant monthly fluctuation. CONCLUSIONS: In high malaria endemic settings with marked variation in seasons of malaria transmission, MOI represents an appropriate malaria metric which provides useful information about the longitudinal changes in malaria transmission in a given area. Besides transmission season, patient age and parasite density are important factors to consider for better understanding of variations in MOI. All allelic families of msp1 and msp2 genes were found in both dry and rainy season. The approach offers the opportunity of translating genotyping data into relevant epidemiological information for malaria control.
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