Publications

@article{Sondo2021-qe,

title = {Polymorphisms in Plasmodium falciparum parasites and mutations in the resistance genes Pfcrt and Pfmdr1 in Nanoro area, Burkina Faso.},

author = {Paul Sondo and Biebo Bihoun and B\'{e}renger Kabore and Marc Christian Tahita and Karim Derra and Toussaint Rouamba and Seydou Nakanabo Diallo and Adama Kazienga and Hamidou Ilboudo and Innocent Valea and Zekiba Tarnagda and Hermann Sorgho and Thierry Lefevre and Halidou Tinto},

doi = {10.11604/pamj.2021.39.118.26959},

issn = {1937-8688},

year  = {2021},

date = {2021-06-10},

urldate = {2021-06-10},

journal = {Pan Afr. Med. J.},

volume = {39},

pages = {118},

publisher = {Pan African Medical Journal},

abstract = {Introduction: from a genetic point of view P. falciparumis 

 extremely polymorphic. There is a variety of parasite strains 

 infesting individuals living in malaria endemic areas. The 

 purpose of this study is to investigate the relationship between 

 polymorphisms in Plasmodium falciparum parasites and Pfcrt and 

 Pfmdr1 gene mutations in Nanoro area, Burkina Faso. Methods: 

 blood samples from plasmodium carriers residing in the Nanoro 

 Health District were genotyped using nested PCR. Parasite gene 

 mutations associated with resistance to antimalarial drugs were 

 detected by PCR-RFLP. Results: samples of 672 patients were 

 successfully genotyped. No msp1and msp2allelic families 

 exhibited an increase in developing mutations in resistance 

 genes. However, mutant strains of these genes were present at 

 greater levels in monoclonal infections than in multi-clonal 

 infections. Conclusion: this study provides an overview of the 

 relationship between polymorphisms in Plasmodium falciparum 

 parasites and mutations in resistance genes. These data will 

 undoubtedly contribute to improving knowledge of the parasite´s 

 biology and its mechanisms of resistance to antimalarial drugs.},

note = {Copyright: Paul Sondo et al.

PMID: 34512854 

PMCID: PMC8396377},

keywords = {Antimalarials/pharmacology, Burkina Faso, Drug Resistance, Falciparum/drug therapy/parasitology, GeneticRestriction Fragment Length, Genotype, Humans, Malaria, Membrane Transport Proteins/genetics, msp1, msp2, Multidrug Resistance-Associated Proteins/genetics, Mutation, Pfcrt, Pfmdr1, Plasmodium falciparum, Plasmodium falciparum/drug effects/genetics/isolation \& purification, Polymerase Chain Reaction, Polymorphism, Protozoan Proteins/genetics},

pubstate = {published},

tppubtype = {article}

}

@article{Sondo2021-at,

title = {Plasmodium falciparum gametocyte carriage in symptomatic  patients shows significant association with genetically diverse  infections, anaemia, and asexual stage density},

author = {Paul Sondo and Biebo Bihoun and Marc Christian Tahita and Karim Derra and Toussaint Rouamba and Seydou Nakanabo Diallo and Adama Kazienga and Hamidou Ilboudo and Innocent Valea and Zekiba Tarnagda and Hermann Sorgho and Thierry Lef`evre and Halidou Tinto},

doi = {10.1186/s12936-020-03559-0},

issn = {1475-2875},

year  = {2021},

date = {2021-01-07},

urldate = {2021-01-01},

journal = {Malar. J.},

volume = {20},

number = {1},

pages = {31},

publisher = {Springer Science and Business Media LLC},

abstract = {BACKGROUND: Multi-genotype malaria infections are frequent in 

 endemic area, and people commonly harbour several genetically 

 distinct Plasmodium falciparum variants. The influence of 

 genetic multiplicity and whether some specific genetic variants 

 are more or less likely to invest into gametocyte production is 

 not clearly understood. This study explored host and 

 parasite-related risk factors for gametocyte carriage, and the 

 extent to which some specific P. falciparum genetic variants are 

 associated with gametocyte carriage. METHODS: Gametocytes and 

 asexual forms were detected by light microscopy on thick smears 

 collected between 2010 and 2012 in Nanoro, Burkina Faso. 

 Merozoite surface protein 1 and 2 were genotyped by nested PCR 

 on clinical samples. Associations between gametocyte carriage 

 and factors, including multiplicity of infection, parasite 

 density, patient age, gender, haemoglobin (Hb) level, and body 

 temperature were assessed. The relationship between the presence 

 of a particular msp1 and msp2 genetic variants and gametocyte 

 carriage was also explored. RESULTS: Of the 724 samples positive 

 to P. falciparum and successfully genotyped, gametocytes were 

 found in 48 samples (6.63%). There was no effect of patient 

 gender, age and body temperature on gametocyte carriage. 

 However, the probability of gametocyte carriage significantly 

 increased with increasing values of multiplicity of infection 

 (MOI). Furthermore, there was a negative association between 

 parasite density and gametocyte carriage. MOI decreased with 

 parasite density in gametocyte-negative patients, but increased 

 in gametocyte carriers. The probability of gametocyte carriage 

 decreased with Hb level. Finally, the genetic composition of the 

 infection influenced gametocyte carriage. In particular, the 

 presence of RO33 increased the odds of developing gametocytes by 

 2 while the other allelic families K1, MAD20, FC27, and 3D7 had 

 no significant impact on the occurrence of gametocytes in 

 infected patients. CONCLUSION: This study provides insight into 

 potential factors influencing gametocyte production in 

 symptomatic patients. The findings contribute to enhance 

 understanding of risk factors associated with gametocyte 

 carriage in humans. Trial registration NCT01232530.},

note = {PMID: 33413393 

PMCID: PMC7791700},

keywords = {Anemia/epidemiology/parasitology, Burkina Faso/epidemiology, Falciparum/epidemiology/parasitology, Gametocyte, Humans, Malaria, msp1, msp2, Multiplicity of infection, Plasmodium   falciparum, Plasmodium falciparum/physiology},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Determinants of Plasmodium falciparum multiplicity of infection and genetic diversity in Burkina Faso.},

author = {Paul Sondo and Karim Derra and Toussaint Rouamba and Seydou Nakanabo Diallo and Paul Taconet and Adama Kazienga and Hamidou Ilboudo and Marc Christian Tahita and Innocent Val\'{e}a and Hermann Sorgho and Thierry Lef\`{e}vre and Halidou Tinto},

year  = {2020},

date = {2020-08-01},

journal = {Parasites \& vectors},

volume = {13},

issue = {1},

pages = {427},

abstract = {BACKGROUND: Investigating malaria transmission dynamics is essential to inform policy decision making. Whether multiplicity of infection (MOI) dynamic from individual infections could be a reliable malaria metric in high transmission settings with marked variation in seasons of malaria transmission has been poorly assessed. This study aimed at investigating factors driving Plasmodium falciparum MOI and genetic diversity in a hyperendemic area of Burkina Faso. METHODS: Blood samples collected from a pharmacovigilance trial were used for polymerase chain reaction genotyping of the merozoite surface proteins 1 and 2. MOI was defined as the number of distinct parasite genotypes co-existing within a particular infection. Monthly rainfall data were obtained from satellite data of the Global Precipitation Measurement Database while monthly malaria incidence aggregated data were extracted from District Health Information Software 2 medical data of the Center-West health regional direction. RESULTS: In the study area, infected people harboured an average of 2.732 (± 0.056) different parasite genotypes. A significant correlation between the monthly MOI and the monthly malaria incidence was observed, suggesting that MOI could be a good predictor of transmission intensity. A strong effect of season on MOI was observed, with infected patients harbouring higher number of parasite genotypes during the rainy season as compared to the dry season. There was a negative relationship between MOI and host age. In addition, MOI decreased with increasing parasite densities, suggesting that there was a within-host competition among co-infecting genetically distinct P. falciparum variants. Each allelic family of the msp1 and msp2 genes was present all year round with no significant monthly fluctuation. CONCLUSIONS: In high malaria endemic settings with marked variation in seasons of malaria transmission, MOI represents an appropriate malaria metric which provides useful information about the longitudinal changes in malaria transmission in a given area. Besides transmission season, patient age and parasite density are important factors to consider for better understanding of variations in MOI. All allelic families of msp1 and msp2 genes were found in both dry and rainy season. The approach offers the opportunity of translating genotyping data into relevant epidemiological information for malaria control.},

keywords = {Age Factors, Antigens, Burkina Faso/epidemiology, Falciparum/epidemiology/parasitology, Genetic Variation, Genotype, Humans, Incidence, Malaria, Merozoite Surface Protein 1/genetics, msp1, msp2, Multiplicity of infection, Parasite Load, Plasmodium falciparum, Plasmodium falciparum/*genetics, Protozoan Proteins/genetics, Protozoan/genetics, Seasons},

pubstate = {MEDLINE},

tppubtype = {article}

}