Publications

@article{Phyo2024,

title = {Intermediate hepatitis C virus (HCV) endemicity and its genotype distribution in Myanmar: A systematic review and meta-analysis},

author = {Zayar Phyo and Ko Ko and Serge Ouoba and Aya Sugiyama and Ulugbek Khudayberdievich Mirzaev and Golda Ataa Akuffo and Chanroth Chhoung and Tomoyuki Akita and Junko Tanaka},

url = {https://pubmed.ncbi.nlm.nih.gov/39298388/},

doi = {10.1371/JOURNAL.PONE.0307872},

issn = {1932-6203},

year  = {2024},

date = {2024-01-01},

journal = {PloS one},

volume = {19},

issue = {9},

publisher = {PLoS One},

abstract = {Background Comprehensive details on Hepatitis C virus (HCV) infection in Myanmar are lacking. This study determined the prevalence of HCV antibodies and ribonucleic acid (RNA) and the distribution of HCV genotypes across different populations in Myanmar from 1990 to 2023. Material and methods A systematic search in PubMed, Web of Science, Scopus, and local journals identified studies reporting on HCV antibodies, RNA, and genotypes, excluding clinical research related to liver disease prognosis. Screening and data extraction was done by two authors and study populations were categorized into low-risk, high-risk, liver disease patients, and refugees outside the country. The pooled prevalence was performed by Dersimonian and Laird method using the R program. The publication bias was shown by funnel plot, the Egger test was used to assess the symmetry of the plot, and the heterogeneity was examined by the Cochran Q test and I2 index. Results Out of 135 reports screened for eligibility, 35 reports comprising 51 studies were included in which 33 studies provided data on HCV seroprevalence in 685,403 individuals, 8 studies reported HCV RNA prevalence in 25,018 individuals, and 10 studies examined HCV genotypes in 1,845 individuals. The pooled seroprevalence of HCV among low-risk, high-risk, liver disease patients and refugees were 2.18%, 37.07%, 33.84%, and 2.52% respectively. HCV RNA-positive rates in these groups were 1.40%, 5.25%, 24.96%, and 0.84% respectively. Seroprevalence studies showed publication bias (Egger test},

keywords = {doi:10.1371/journal.pone.0307872, Endemic Diseases, Genotype*, Hepacivirus* / genetics, Hepacivirus* / isolation \& purification, Hepatitis C* / epidemiology, Hepatitis C* / virology, Humans, Junko Tanaka, Ko Ko, MEDLINE, Meta-Analysis, Myanmar / epidemiology, National Center for Biotechnology Information, National Institutes of Health, National Library of Medicine, NCBI, NIH, NLM, PMC11412534, pmid:39298388, Prevalence, PubMed Abstract, RNA, Seroepidemiologic Studies, Systematic review, Viral / genetics, Zayar Phyo},

pubstate = {published},

tppubtype = {article}

}

@article{Post2021-oo,

title = {Altered ex-vivo cytokine responses in children with asymptomatic  Plasmodium falciparum infection in Burkina Faso: An additional  argument to treat asymptomatic malaria?},

author = {Annelies Post and Berenger Kabor\'{e} and Mike Berendsen and Salou Diallo and Ousmane Traore and Rob J W Arts and Mihai G Netea and Leo A B Joosten and Halidou Tinto and Jan Jacobs and Quirijn Mast and Andr\'{e} Ven},

doi = {10.3389/fimmu.2021.614817},

issn = {1664-3224},

year  = {2021},

date = {2021-06-09},

urldate = {2021-06-09},

journal = {Front. Immunol.},

volume = {12},

pages = {614817},

publisher = {Frontiers Media SA},

abstract = {Introduction: Patients with clinical malaria have an increased 

 risk for bacterial bloodstream infections. We hypothesized that 

 asymptomatic malaria parasitemia increases susceptibility for 

 bacterial infections through an effect on the innate immune 

 system. We measured circulating cytokine levels and ex-vivo 

 cytokine production capacity in asymptomatic malaria and 

 compared with controls. Methods: Data were collected from 

 asymptomatic participants \<5 years old with and without positive 

 malaria microscopy, as well as from hospitalized patients \<5 

 years old with clinical malaria, bacteremia, or 

 malaria/bacteremia co-infections in a malaria endemic region of 

 Burkina Faso. Circulating cytokines (TNF-$alpha$, IFN-$gamma$, 

 IL-6, IL-10) were measured using multiplex assays. Whole blood 

 from asymptomatic participants with and without positive malaria 

 microscopy were ex-vivo stimulated with S. aureus, E. coli LPS 

 and Salmonella Typhimurium; cytokine concentrations 

 (TNF-$alpha$, IFN-$gamma$, IL-1$beta$, IL-6, IL-10) were 

 measured on supernatants using ELISA. Results: Included were children with clinical malaria (n=118), bacteremia (n=22), malaria and bacteremia co-infection (n=9), asymptomatic malaria (n=125), and asymptomatic controls (n=237). Children with either 

 clinical or asymptomatic malaria had higher plasma cytokine 

 concentrations than controls. Cytokine concentrations correlated 

 positively with malaria parasite density with the strongest correlation for IL-10 in both asymptomatic (r=0.63) and clinical malaria (r=0.53). Patients with bacteremia had lower circulating 

 IL-10, TNF-$alpha$ and IFN-$gamma$ and higher IL-6 

 concentrations, compared to clinical malaria. Ex-vivo whole 

 blood cytokine production to LPS and S. aureus was significantly 

 lower in asymptomatic malaria compared to controls. Whole blood 

 IFN-$gamma$ and IL-10 production in response to Salmonella was 

 also lower in asymptomatic malaria. Interpretation: In children 

 with asymptomatic malaria, cytokine responses upon ex-vivo 

 bacterial stimulation are downregulated. Further studies are 

 needed to explore if the suggested impaired innate immune 

 response to bacterial pathogens also translates into impaired 

 control of pathogens such as Salmonella spp.},

note = {Copyright © 2021 Post, Kabor\'{e}, Berendsen, Diallo, Traore, Arts, Netea, Joosten, Tinto, Jacobs, de Mast and van der Ven.

PMID: 34177883 

PMCID: PMC8220162},

keywords = {Asymptomatic Diseases, asymptomatic malaria, bacteraemia, Bacteremia, bloodstream infection, Burkina Faso/epidemiology, Cells, Child, Cultured, Cytokines/metabolism, Endemic Diseases, Female, Humans, Infant, iNTS, Lipopolysaccharides/immunology, Malaria/epidemiology/immunology, Male, Parasite Load, Plasmodium falciparum/physiology, Preschool, Salmonella},

pubstate = {published},

tppubtype = {article}

}