| Charlie Franck Alfred Compaoré, Jacques Kaboré, Hamidou Ilboudo, Lian Francesca Thomas, Laura Cristina Falzon, Mohamed Bamba, Hassane Sakande, Minayégninrin Koné, Dramane Kaba, Clarisse Bougouma, Ilboudo Adama, Ouedraogo Amathe, Adrien Marie Gaston Belem, Eric Maurice Fèvre, Philippe Büscher, Veerle Lejon, Vincent Jamonneau Monitoring the elimination of gambiense human African trypanosomiasis in the historical focus of Batié, South–West Burkina Faso Journal Article In: Parasite, vol. 29, pp. 25, 2022, ISSN: 1776-1042. @article{nokey,
title = {Monitoring the elimination of \textit{gambiense} human African trypanosomiasis in the historical focus of Bati\'{e}, South\textendashWest Burkina Faso},
author = {Charlie Franck Alfred Compaor\'{e} and Jacques Kabor\'{e} and Hamidou Ilboudo and Lian Francesca Thomas and Laura Cristina Falzon and Mohamed Bamba and Hassane Sakande and Minay\'{e}gninrin Kon\'{e} and Dramane Kaba and Clarisse Bougouma and Ilboudo Adama and Ouedraogo Amathe and Adrien Marie Gaston Belem and Eric Maurice F\`{e}vre and Philippe B\"{u}scher and Veerle Lejon and Vincent Jamonneau},
url = {https://www.parasite-journal.org/10.1051/parasite/2022024},
doi = {10.1051/parasite/2022024},
issn = {1776-1042},
year = {2022},
date = {2022-01-01},
journal = {Parasite},
volume = {29},
pages = {25},
abstract = {\<p\> The World Health Organisation has targeted the elimination of human African trypanosomiasis (HAT) as zero transmission by 2030. Continued surveillance needs to be in place for early detection of re-emergent cases. In this context, the performance of diagnostic tests and testing algorithms for detection of the re-emergence of \<italic\>Trypanosoma brucei gambiense\</italic\> HAT remains to be assessed. We carried out a door-to-door active medical survey for HAT in the historical focus of Bati\'{e}, South\textendashWest Burkina Faso. Screening was done using three rapid diagnostic tests (RDTs). Two laboratory tests (ELISA/ \<italic\>T. b. gambiense\</italic\> and immune trypanolysis) and parasitological examination were performed on RDT positives only. In total, 5883 participants were screened, among which 842 (14%) tested positive in at least one RDT. Blood from 519 RDT positives was examined microscopically but no trypanosomes were observed. The HAT Sero- \<italic\>K\</italic\> -Set test showed the lowest specificity of 89%, while the specificities of SD Bioline HAT and rHAT Sero-Strip were 92% and 99%, respectively. The specificity of ELISA/ \<italic\>T. b. gambiense\</italic\> and trypanolysis was 99% (98\textendash99%) and 100% (99\textendash100%), respectively. Our results suggest that \<italic\>T. b. gambiense\</italic\> is no longer circulating in the study area and that zero transmission has probably been attained. While a least cost analysis is still required, our study showed that RDT preselection followed by trypanolysis may be a useful strategy for post-elimination surveillance in Burkina Faso. \</p\>},
keywords = {Burkina Faso, Diagnosis, Dried blood spot, Elimination, Human African trypanosomiasis, Rapid diagnostic test, Specificity, Trypanosoma brucei gambiense},
pubstate = {published},
tppubtype = {article}
}
<p> The World Health Organisation has targeted the elimination of human African trypanosomiasis (HAT) as zero transmission by 2030. Continued surveillance needs to be in place for early detection of re-emergent cases. In this context, the performance of diagnostic tests and testing algorithms for detection of the re-emergence of <italic>Trypanosoma brucei gambiense</italic> HAT remains to be assessed. We carried out a door-to-door active medical survey for HAT in the historical focus of Batié, South–West Burkina Faso. Screening was done using three rapid diagnostic tests (RDTs). Two laboratory tests (ELISA/ <italic>T. b. gambiense</italic> and immune trypanolysis) and parasitological examination were performed on RDT positives only. In total, 5883 participants were screened, among which 842 (14%) tested positive in at least one RDT. Blood from 519 RDT positives was examined microscopically but no trypanosomes were observed. The HAT Sero- <italic>K</italic> -Set test showed the lowest specificity of 89%, while the specificities of SD Bioline HAT and rHAT Sero-Strip were 92% and 99%, respectively. The specificity of ELISA/ <italic>T. b. gambiense</italic> and trypanolysis was 99% (98–99%) and 100% (99–100%), respectively. Our results suggest that <italic>T. b. gambiense</italic> is no longer circulating in the study area and that zero transmission has probably been attained. While a least cost analysis is still required, our study showed that RDT preselection followed by trypanolysis may be a useful strategy for post-elimination surveillance in Burkina Faso. </p> |
 | Olivier Fataki Asina, Harry Noyes, Bruno Bucheton, Hamidou Ilboudo, Annette MacLeod, Dieudonné Mumba Ngoyi SNPs in IL4 and IFNG show no protective associations with human African trypanosomiasis in the Democratic Republic of the Congo: a case-control study. Journal Article In: AAS open research, vol. 3, pp. 35, 2020, ISSN: 2515-9321. @article{nokey,
title = {SNPs in IL4 and IFNG show no protective associations with human African trypanosomiasis in the Democratic Republic of the Congo: a case-control study.},
author = {Olivier Fataki Asina and Harry Noyes and Bruno Bucheton and Hamidou Ilboudo and Annette MacLeod and Dieudonn\'{e} Mumba Ngoyi},
doi = {10.12688/aasopenres.12999.1},
issn = {2515-9321},
year = {2020},
date = {2020-01-01},
urldate = {2020-01-01},
journal = {AAS open research},
volume = {3},
pages = {35},
abstract = {Background: Human African trypanosomiasis (HAT) is a protozoal disease transmitted by tsetse flies. Infection with trypanosomes can lead directly to active HAT or latent infection with no detectable parasites, which may progress to active HAT or to spontaneous self-cure. Genetic variation could explain these differences in the outcome of infection. To test this hypothesis, polymorphisms in 17 candidate genes were tested ( APOL1 [ G1 and G2], CFH, HLA-A, HPR, HP, IL1B, IL12B, IL12RB1, IL10, IL4R, MIF, TNFA , IL6, IL4, IL8, IFNG, and HLA-G). Methods: Samples were collected in Democratic Republic of the Congo. 233 samples were genotyped: 100 active HAT cases, 33 from subjects with latent infections and 100 negative controls. Commercial service providers genotyped polymorphisms at 96 single nucleotide polymorphisms (SNPs) on 17 genes. Data were analyzed using Plink V1.9 software and R. Loci, with suggestive associations (uncorrected p \< 0.05) validated using an additional 594 individuals, including 164 cases and 430 controls. Results: After quality control, 87 SNPs remained in the analysis. Two SNPs in IL4 and two in IFNG were suggestively associated (uncorrected p\<0.05) with a differential risk of developing a Trypanosoma brucei gambiense infection in the Congolese population. The IFNG minor allele (rs2430561, rs2069718) SNPs were protective in comparison between latent infections and controls. Carriers of the rs2243258_T and rs2243279_A alleles of IL4 and the rs2069728_T allele of IFNG had a reduced risk of developing illness or latent infection, respectively. None of these associations were significant after Bonferroni correction for multiple testing. A validation study using more samples was run to determine if the absence of significant association was due to lack of power. Conclusions: This study showed no evidence of an association of HAT with IL4 and IFNG SNPs or with APOL1 G1 and G2 alleles, which have been found to be protective in other studies.},
keywords = {genes, Human African trypanosomiasis, polymorphisms, T.b.gambiense},
pubstate = {published},
tppubtype = {article}
}
Background: Human African trypanosomiasis (HAT) is a protozoal disease transmitted by tsetse flies. Infection with trypanosomes can lead directly to active HAT or latent infection with no detectable parasites, which may progress to active HAT or to spontaneous self-cure. Genetic variation could explain these differences in the outcome of infection. To test this hypothesis, polymorphisms in 17 candidate genes were tested ( APOL1 [ G1 and G2], CFH, HLA-A, HPR, HP, IL1B, IL12B, IL12RB1, IL10, IL4R, MIF, TNFA , IL6, IL4, IL8, IFNG, and HLA-G). Methods: Samples were collected in Democratic Republic of the Congo. 233 samples were genotyped: 100 active HAT cases, 33 from subjects with latent infections and 100 negative controls. Commercial service providers genotyped polymorphisms at 96 single nucleotide polymorphisms (SNPs) on 17 genes. Data were analyzed using Plink V1.9 software and R. Loci, with suggestive associations (uncorrected p < 0.05) validated using an additional 594 individuals, including 164 cases and 430 controls. Results: After quality control, 87 SNPs remained in the analysis. Two SNPs in IL4 and two in IFNG were suggestively associated (uncorrected p<0.05) with a differential risk of developing a Trypanosoma brucei gambiense infection in the Congolese population. The IFNG minor allele (rs2430561, rs2069718) SNPs were protective in comparison between latent infections and controls. Carriers of the rs2243258_T and rs2243279_A alleles of IL4 and the rs2069728_T allele of IFNG had a reduced risk of developing illness or latent infection, respectively. None of these associations were significant after Bonferroni correction for multiple testing. A validation study using more samples was run to determine if the absence of significant association was due to lack of power. Conclusions: This study showed no evidence of an association of HAT with IL4 and IFNG SNPs or with APOL1 G1 and G2 alleles, which have been found to be protective in other studies. |
