Publications

@article{Soumah2024,

title = {Prevalence of dermal trypanosomes in suspected and confirmed cases of gambiense human African trypanosomiasis in Guinea},

author = {Alseny M’Mah Soumah and Mariame Camara and Justin Windingoudi Kabor\'{e} and Ibrahim Sadissou and Hamidou Ilboudo and Christelle Travaill\'{e} and Oumou Camara and Magali Tichit and Jacques Kabor\'{e} and Salimatou Boiro and Aline Crouzols and Jean Marc Tsagmo Ngoune and David Hardy and A\"{i}ssata Camara and Vincent Jamonneau and Annette Macleod and Jean Mathieu Bart and Mamadou Camara and Bruno Bucheton and Brice Rotureau},

url = {https://pubmed.ncbi.nlm.nih.gov/39159265/},

doi = {10.1371/JOURNAL.PNTD.0012436},

issn = {1935-2735},

year  = {2024},

date = {2024-01-01},

journal = {PLoS neglected tropical diseases},

volume = {18},

issue = {8},

publisher = {PLoS Negl Trop Dis},

abstract = {The skin is an anatomical reservoir for African trypanosomes, yet the prevalence of extravascular parasite carriage in the population at risk of gambiense Human African Trypanosomiasis (gHAT) remains unclear. Here, we conducted a prospective observational cohort study in the HAT foci of Forecariah and Boffa, Republic of Guinea. Of the 18,916 subjects serologically screened for gHAT, 96 were enrolled into our study. At enrolment and follow-up visits, participants underwent a dermatological examination and had blood samples and superficial skin snip biopsies taken for examination by molecular and immuno-histological methods. In seropositive individuals, dermatological symptoms were significantly more frequent as compared to seronegative controls. Trypanosoma brucei DNA was detected in the blood of 67% of confirmed cases (22/33) and 9% of unconfirmed seropositive individuals (3/32). However, parasites were detected in the extravascular dermis of up to 71% of confirmed cases (25/35) and 41% of unconfirmed seropositive individuals (13/32) by PCR and/or immuno-histochemistry. Six to twelve months after treatment, trypanosome detection in the skin dropped to 17% of confirmed cases (5/30), whereas up to 25% of unconfirmed, hence untreated, seropositive individuals (4/16) were still found positive. Dermal trypanosomes were observed in subjects from both transmission foci, however, the occurrence of pruritus and the PCR positivity rates were significantly higher in unconfirmed seropositive individuals in Forecariah. The lower sensitivity of superficial skin snip biopsies appeared critical for detecting trypanosomes in the basal dermis. These results are discussed in the context of the planned elimination of gHAT.},

keywords = {Adolescent, Adult, African* / diagnosis, African* / epidemiology, African* / parasitology, Alseny M'mah Soumah, Brice Rotureau, Child, DNA, doi:10.1371/journal.pntd.0012436, Female, Guinea / epidemiology, Humans, Male, Mariame Camara, MEDLINE, Middle Aged, National Center for Biotechnology Information, National Institutes of Health, National Library of Medicine, NCBI, NIH, NLM, Observational Study, PMC11361743, pmid:39159265, Prevalence, Prospective Studies, Protozoan / genetics, PubMed Abstract, Skin* / parasitology, Skin* / pathology, Trypanosoma brucei gambiense* / isolation \& purification, Trypanosomiasis, Young Adult},

pubstate = {published},

tppubtype = {article}

}

@article{Ouoba2023,

title = {Intermediate hepatitis B virus infection prevalence among 1622 pregnant women in rural Burkina Faso and implications for mother-to-child transmission},

author = {Serge Ouoba and Ko Ko and Moussa Lingani and Shintaro Nagashima and Alice N. Guingan\'{e} and E. Bunthen and Md Razeen Ashraf Hussain and Aya Sugiyama and Tomoyuki Akita and Masayuki Ohisa and Moussa Abdel Sanou and Ousmane Traore and Job Wilfried Nassa and Maimouna Sanou and Kazuaki Takahashi and Halidou Tinto and Junko Tanaka},

url = {https://pubmed.ncbi.nlm.nih.gov/37059812/},

doi = {10.1038/S41598-023-32766-3},

issn = {2045-2322},

year  = {2023},

date = {2023-01-01},

journal = {Scientific reports},

volume = {13},

issue = {1},

publisher = {Sci Rep},

abstract = {In highly endemic countries for hepatitis B virus (HBV) infection, childhood infection, including mother-to-child transmission (MTCT), represents the primary transmission route. High maternal DNA level (viral load ≥ 200,000 IU/mL) is a significant factor for MTCT. We investigated the prevalence of HBsAg, HBeAg, and high HBV DNA among pregnant women in three hospitals in Burkina Faso and assessed the performance of HBeAg to predict high viral load. Consenting pregnant women were interviewed on their sociodemographic characteristics and tested for HBsAg by a rapid diagnostic test, and dried blood spot (DBS) samples were collected for laboratory analyses. Of the 1622 participants, HBsAg prevalence was 6.5% (95% CI, 5.4\textendash7.8%). Among 102 HBsAg-positive pregnant women in DBS samples, HBeAg was positive in 22.6% (95% CI, 14.9\textendash31.9%), and viral load was quantified in 94 cases, with 19.1% having HBV DNA ≥ 200,000 IU/mL. HBV genotypes were identified in 63 samples and predominant genotypes were E (58.7%) and A (36.5%). The sensitivity of HBeAg by using DBS samples to identify high viral load in the 94 cases was 55.6%, and the specificity was 86.8%. These findings highlight the need to implement routine HBV screening and effective MTCT risk assessment for all pregnant women in Burkina Faso to enable early interventions that can effectively reduce MTCT.},

keywords = {Burkina Faso / epidemiology, Child, DNA, doi:10.1038/s41598-023-32766-3, Female, Hepatitis B e Antigens, Hepatitis B Surface Antigens, Hepatitis B virus / genetics, Hepatitis B* / diagnosis, Humans, Infectious Disease Transmission, Infectious* / epidemiology, Junko Tanaka, Ko Ko, MEDLINE, National Center for Biotechnology Information, National Institutes of Health, National Library of Medicine, NCBI, NIH, NLM, Non-U.S. Gov't, PMC10103033, pmid:37059812, Pregnancy, Pregnancy Complications, Pregnant Women, Prevalence, PubMed Abstract, Research Support, Serge Ouoba, Vertical / prevention \& control, Viral / genetics},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Analytical sensitivity of loopamp and quantitative real-time PCR on dried blood spots and their potential role in monitoring human African trypanosomiasis elimination.},

author = {Charlie Franck Alfred Compaor\'{e} and Hamidou Ilboudo and Jacques Kabor\'{e} and Justin Windingoudi Kabor\'{e} and Oumou Camara and Mohamed Bamba and Hassane Sakande and Minay\'{e}gninrin Kon\'{e} and Mamadou Camara and Dramane Kaba and Adrien Marie Gaston Belem and Stijn Deborggraeve and Philippe B\"{u}scher and Bruno Bucheton and Veerle Lejon and Vincent Jamonneau},

doi = {10.1016/j.exppara.2020.108014},

issn = {1090-2449 0014-4894},

year  = {2020},

date = {2020-12-01},

urldate = {2020-12-01},

journal = {Experimental parasitology},

volume = {219},

pages = {108014},

address = {United States},

abstract = {The objective set by WHO to reach elimination of human African trypanosomiasis (HAT) as a public health problem by 2020 is being achieved. The next target is the interruption of gambiense-HAT transmission in humans by 2030. To monitor progress towards this target, in areas where specialized local HAT control capacities will disappear, is a major challenge. Test specimens should be easily collectable and safely transportable such as dried blood spots (DBS). Monitoring tests performed in regional reference centres should be reliable, cheap and allow analysis of large numbers of specimens in a high-throughput format. The aim of this study was to assess the analytical sensitivity of Loopamp, M18S quantitative real-time PCR (M18S qPCR) and TgsGP qPCR as molecular diagnostic tests for the presence of Trypanosoma brucei gambiense in DBS. The sensitivity of the Loopamp test, with a detection limit of 100 trypanosomes/mL, was in the range of parasitaemias commonly observed in HAT patients, while detection limits for M18S and TgsGP qPCR were respectively 1000 and 10,000 trypanosomes/mL. None of the tests was entirely suitable for high-throughput use and further development and implementation of sensitive high-throughput molecular tools for monitoring HAT elimination are needed.},

keywords = {African/blood/diagnosis/*prevention \& control, Algorithms, Animals, Blood Specimen Collection/methods/standards, Diagnosis, DNA, Dried blood spots, Feasibility, High-Throughput Screening Assays/methods/standards, Humans, Loopamp, Mice, Molecular Diagnostic Techniques/*standards, Nucleic Acid Amplification Techniques/*standards, Protozoan/isolation \& purification, Quantitative real-time PCR, Real-Time Polymerase Chain Reaction/methods/*standards, Sensitivity, Sensitivity and Specificity, Specimen Handling/methods/standards, Trypanosoma brucei gambiense, Trypanosoma brucei gambiense/genetics/*isolation \& purification, Trypanosomiasis},

pubstate = {published},

tppubtype = {article}

}