 | Charlie Franck Alfred Compaoré, Hamidou Ilboudo, Jacques Kaboré, Justin Windingoudi Kaboré, Oumou Camara, Mohamed Bamba, Hassane Sakande, Minayégninrin Koné, Mamadou Camara, Dramane Kaba, Adrien Marie Gaston Belem, Stijn Deborggraeve, Philippe Büscher, Bruno Bucheton, Veerle Lejon, Vincent Jamonneau Analytical sensitivity of loopamp and quantitative real-time PCR on dried blood spots and their potential role in monitoring human African trypanosomiasis elimination. Journal Article In: Experimental parasitology, vol. 219, pp. 108014, 2020, ISSN: 1090-2449 0014-4894. @article{nokey,
title = {Analytical sensitivity of loopamp and quantitative real-time PCR on dried blood spots and their potential role in monitoring human African trypanosomiasis elimination.},
author = {Charlie Franck Alfred Compaor\'{e} and Hamidou Ilboudo and Jacques Kabor\'{e} and Justin Windingoudi Kabor\'{e} and Oumou Camara and Mohamed Bamba and Hassane Sakande and Minay\'{e}gninrin Kon\'{e} and Mamadou Camara and Dramane Kaba and Adrien Marie Gaston Belem and Stijn Deborggraeve and Philippe B\"{u}scher and Bruno Bucheton and Veerle Lejon and Vincent Jamonneau},
doi = {10.1016/j.exppara.2020.108014},
issn = {1090-2449 0014-4894},
year = {2020},
date = {2020-12-01},
urldate = {2020-12-01},
journal = {Experimental parasitology},
volume = {219},
pages = {108014},
address = {United States},
abstract = {The objective set by WHO to reach elimination of human African trypanosomiasis (HAT) as a public health problem by 2020 is being achieved. The next target is the interruption of gambiense-HAT transmission in humans by 2030. To monitor progress towards this target, in areas where specialized local HAT control capacities will disappear, is a major challenge. Test specimens should be easily collectable and safely transportable such as dried blood spots (DBS). Monitoring tests performed in regional reference centres should be reliable, cheap and allow analysis of large numbers of specimens in a high-throughput format. The aim of this study was to assess the analytical sensitivity of Loopamp, M18S quantitative real-time PCR (M18S qPCR) and TgsGP qPCR as molecular diagnostic tests for the presence of Trypanosoma brucei gambiense in DBS. The sensitivity of the Loopamp test, with a detection limit of 100 trypanosomes/mL, was in the range of parasitaemias commonly observed in HAT patients, while detection limits for M18S and TgsGP qPCR were respectively 1000 and 10,000 trypanosomes/mL. None of the tests was entirely suitable for high-throughput use and further development and implementation of sensitive high-throughput molecular tools for monitoring HAT elimination are needed.},
keywords = {African/blood/diagnosis/*prevention \& control, Algorithms, Animals, Blood Specimen Collection/methods/standards, Diagnosis, DNA, Dried blood spots, Feasibility, High-Throughput Screening Assays/methods/standards, Humans, Loopamp, Mice, Molecular Diagnostic Techniques/*standards, Nucleic Acid Amplification Techniques/*standards, Protozoan/isolation \& purification, Quantitative real-time PCR, Real-Time Polymerase Chain Reaction/methods/*standards, Sensitivity, Sensitivity and Specificity, Specimen Handling/methods/standards, Trypanosoma brucei gambiense, Trypanosoma brucei gambiense/genetics/*isolation \& purification, Trypanosomiasis},
pubstate = {published},
tppubtype = {article}
}
The objective set by WHO to reach elimination of human African trypanosomiasis (HAT) as a public health problem by 2020 is being achieved. The next target is the interruption of gambiense-HAT transmission in humans by 2030. To monitor progress towards this target, in areas where specialized local HAT control capacities will disappear, is a major challenge. Test specimens should be easily collectable and safely transportable such as dried blood spots (DBS). Monitoring tests performed in regional reference centres should be reliable, cheap and allow analysis of large numbers of specimens in a high-throughput format. The aim of this study was to assess the analytical sensitivity of Loopamp, M18S quantitative real-time PCR (M18S qPCR) and TgsGP qPCR as molecular diagnostic tests for the presence of Trypanosoma brucei gambiense in DBS. The sensitivity of the Loopamp test, with a detection limit of 100 trypanosomes/mL, was in the range of parasitaemias commonly observed in HAT patients, while detection limits for M18S and TgsGP qPCR were respectively 1000 and 10,000 trypanosomes/mL. None of the tests was entirely suitable for high-throughput use and further development and implementation of sensitive high-throughput molecular tools for monitoring HAT elimination are needed. |
